Like most MMORPGs, gameplay centers on venturing into dungeons and combating monsters in real-time. The players combat monsters and complete quests, in the process acquiring in-game currency, called "Mesos", experience points (EXP), and various items. Players can kill monsters alone, or they can form a party with up to six total characters. Loot is shared based on relative damage and level of characters in the party, more being awarded to the higher-level members.

MapleStory's 2D scrolling viewpoint more closely resembles a platformer rather than the typical 3D environment or top-down view of other games, such as Guild Wars or Mu Online. The controls for the game are executed using the keyboard and mouse. The keyboard is used for many game functions, and much of it can be rearranged to suit users' needs. The mouse is mainly used to trigger non-player characters and manipulate items.

maple story mesos characters exist in "worlds" or "servers." Players are allowed to create multiple characters in each world. Each world, similar in content between each other in the same version, is split into at most twenty channels, among which characters are allowed to freely switch. The ability to transfer entire characters between worlds was added in December 2007 to GlobalMS. However, this feature has restrictions — transfer of mesos is limited to 1 million, and players cannot transfer to the newest world.